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Using The HIROX/FMP Imaging and Harvesting
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These application notes describe the use of the HIROX/FMP imaging
and harvesting Digital 3D microscope with either 24, 96, 384, 1536 or other
crystalization plates.
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1
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First, scan or enter plate information with the system. Insert
plate onto plate specific holder.
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2
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Load plate with sample to be harvested onto automated XY stage. If
sample is a cover slip, load coverslip onto special cover slip transporter.
If well is covered, uncover it.
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3
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Enter well # (A1, B43, etc.) into software.
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4
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System moves plate to proper well # location.
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5
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Without rotationg lens adapter, view content of well. If possible,
remove crystal, freeze and recover well.
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6
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If crystal position is difficult to see or work with, remove the
plate and install rotating adapter (Item# AD-5040RLS25, AD-5040RLS45,
AD-5040RVS).
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7
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Reload the plate as in step (2-4) above.
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8
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Re-focus and adjust rotating lens position until best view is
achieved of the crystal.
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9
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Remove crystal, freeze and replace coverslip.
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Notes: This system works under 0°C- 40 °C,
25%-85% humidity.
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Lysozyme
(3-D 150x)
(35° Oblique
Angle)
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Lysozyme
(3-D 150x)
(35° Oblique
Angle)
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Lysozyme
(3-D 150x)
(55° Oblique
Angle)
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Lysozyme
(150x Multi Focus 4up)
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Crystal
(150x)
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Crystal
(300x)
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Lysozyme
(50x-250x 4up)
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Lysozyme
(250x)
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Lysozyme
(3-D 150x)
(25° Oblique
Angle)
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*Lysozyme was contributed by Schering-Plough Research Institute
in Kenilworth, NJ
Researcher: Paul Reichert
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Lysozyme
(200x)
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Lysozyme
(400x)
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Salt
(3-D 50x-200x)
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 return to top
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