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The short
answer is NO, 300x in a digital microscope is not the same as 300x in a
conventional microscope. Just as the magnification of an image photographed
in a conventional microscope depends on the size of the final print so too
the magnification of a digital microscope image will be determined by the
size of the monitor on which it is displayed. With digital microscopy it is
really more relevant to talk about Field of View (FOV) rather than
magnification - a 500-micrometer sample viewed on a 15" computer
display is much lower "mag" than the same sample viewed on a
large screen with a video projector. The question is to be asked is -
"What is the ratio of the size of the real object to its size in the
digital image?" Generally, with a 15” to 17” monitor this works out to be roughly 40%
to 60% of the magnification you would be accustomed to in a conventional
microscope. That is, 1000x in a digital microscope would be approximately
the same as 400x to 600x in a conventional microscope. The HiScope nominal
magnifications are given for display on a standard 17" computer
monitor. Table 1 gives a range of FOV for different magnifications.
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